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Genetic association and computational analysis of <i>CYP2R1</i> gene polymorphisms rs2060793 and rs12794714 with vitamin D deficiency and acute myocardial infarction in the Bangladeshi population: A case control study

by Sadia Akter, Md. Nazid Bin Ibrahim, Zimam Mahmud, Sonia Tamanna, Md. Shakhawat Hossain Shawon, Farzana Ansari, Md. Zakir Hossain Howlader

Acute myocardial infarction (AMI) remains a leading cause of cardiovascular morbidity and mortality worldwide. Emerging evidence highlights vitamin D as a critical determinant of cardiovascular health. The CYP2R1 gene encodes the key 25-hydroxylase enzyme responsible for converting vitamin D to its principal circulating metabolite, 25-hydroxyvitamin D. However, the influence of CYP2R1 polymorphisms on AMI susceptibility, particularly within South Asian populations, has not been well characterized. This study investigates the association of two CYP2R1 variants, rs2060793 and rs12794714, with AMI risk and their relationship with serum vitamin D levels in a Bangladeshi cohort. A total of 502 participants comprising 251 AMI patients and 251 age- and sex-matched controls were analyzed. Genomic DNA was extracted and genotyped using PCR-RFLP, while serum 25-hydroxyvitamin D3 levels were quantified by HPLC. AMI patients exhibited markedly lower vitamin D concentrations (23.92 ± 0.94 ng/mL) than controls (30.3 ± 0.86 ng/mL; p p = 0.0064). The dominant model (TC + CC vs. TT) further confirmed this relationship (OR = 2.53, 95% CI: 1.39–4.61, p = 0.0016). In contrast, rs12794714 showed no significant association with AMI in this population. Stratified analysis indicated that rs2060793 was significantly linked to AMI in males but not females, while both variants were associated with increased risk in individuals aged ≤60 years, but not in those >60 years. Bioinformatic and molecular docking analyses (RegulomeDB, JASPAR, HADDOCK 2.4, DNAproDB) further demonstrated potential regulatory effects of these variants on CYP2R1 function. Collectively, our findings reveal a novel association between CYP2R1 rs2060793 and vitamin D deficiency with AMI risk in the Bangladeshi population, underscoring the interplay of genetic and metabolic determinants in the molecular pathogenesis of AMI.

Factors associated with high viral load among HIV clients aged 15 years and older receiving treatment in Tanga city council, Tanzania: A facility-based cross-sectional study

by Aqbara Ibrahim Chande, Novatus A. Tesha, Bruno Sunguya

Background

High viral load indicates poor treatment outcomes among people living with HIV (PLHIV) on antiretroviral therapy (ART). However, there is a dearth of evidence on specific factors associated with high viral load in resource-limited settings, including Tanzania.

Aim

The aim of this study is to identify factors contributing to high viral load among PLHIV aged 15 years and older on ART for at least six months in Tanga, Tanzania.

Methods

This is analytical cross-sectional study of 233 PLHIV attending the Care and Treatment Centre (CTC) in Tanga region from September to November 2023. A systematic sampling method was used to select participants for face-to-face interviews. A structured questionnaire was used to collect socio-demographic information while clinical data were collected from the patients’ records and CTC database. Descriptive analysis was used to estimate the prevalence of high viral load, while Pearson Chi-square tests compared categorical variables, and the logistic regression assessed determinants of high viral load.

Results

High viral load was prevalent among 35.2% [95% CI: 29.3%−41.6%] PLHIV attending CTC in Tanga region. Higher viral load was noted among younger adults (52.5%), those in sales/services (63.6%), professionals (54.5%), and unskilled workers (53.2%) compared to their counterparts. PLHIV with severe food insecurity were more likely to exhibit higher viral load compared to those from food secure households (AOR = 9.6; 95% CI: 2.6–35.2). Those consuming alcohol were 4.2 times more likely to have a higher viral load compared to non-drinkers (AOR = 4.2; 95% CI: 1.4–12.5). PLHIV aged 20–24 were 5.4 times more likely to exhibit elevated viral load levels compared to their older counterparts (AOR = 5.4; 95% CI: 1.8–15.7). Additionally, PLHIV in sales and services occupations were 6.1 times more likely to have a higher viral load compared to those in agriculture (AOR = 6.1; 95% CI: 1.1–35 and those facing high stigma were 4.3 times more likely to have a higher HVL than individuals with good social support and low stigma (AOR = 4.3, 95% CI: 1.0–18.7, p = 0.049).).

Conclusion

More than one in three adult PLHIV on ART in Tanga Tanzania had a high viral load. This burden highlights a steep climb to reach the last 95 target ahead of the deadline. Efforts should focus on young adults, those with households’ food insecurity, consuming alcohol, and with perceived stigma in Tanzania and areas with similar context.

Candida Drug Resistance in Patients With Diabetic Foot Ulcers: A Systematic Review and Meta‐Analysis

ABSTRACT

Diabetic foot ulcers (DFUs) are one of the most serious and common complications that, if not treated properly, can lead to potential damage and even amputation. The aim of this systematic review and meta-analysis was to assess the drug-resistant Candida species in DFU. PubMed, Web of Science, Scopus and Google Scholar databases were systematically searched for eligible articles up to 22 June 2024. All articles on Candida diabetic foot infections that reported data on drug resistance were included in the study. In addition to general information, data on the type and number of fungi and the percentage of resistance to each drug were collected for analysis. A total of 238 studies were screened and finally, 16 articles were selected and analysed. Candida albicans was the most frequently isolated species in DFUs, followed by Candida tropicalis and Candida parapsilosis. For antifungal agents, the highest resistance was reported to Nystatin (32.48%, p-value = 0.30), Itraconazole (19.46%, p-value = 0.001) and Fluconazole (16.4%, p-value = 0.001). Miconazole (1.18%, p-value = 0.54) and Caspofungin (4.69%, p-value = 0.01) had the lowest resistance rates. For all drugs, resistance was higher in C. albicans than in non-albicans. This study found that antifungal drug resistance in Candida species is high in patients with DFUs, especially to itraconazole and fluconazole. Caspofungin, micafungin and voriconazole were more effective. Antifungal treatment in these patients should prioritize agents with lower resistance rates to improve outcomes and reduce the risk of treatment failure.

Protocol Registration: PROSPERO—CRD42024567133.

Toward Health System Reform: Unveiling the Relationship Between Nurses' Psychological Empowerment and Job Embeddedness Under the Umbrella of the New Universal Health Insurance System

ABSTRACT

Background

The new Universal Health Insurance (UHI) reforms aim to improve equity and quality of healthcare delivery; however, their sustainability depends on engagement and retention of the nursing workforce. Psychological empowerment has been identified as a key factor influencing nurses' attitudes and work-related behaviors, yet the evidence on how it relates to job embeddedness among nurses working in the early phase of UHI implementation in Egypt remains underexplored.

Aim

This study aimed to assess the association between psychological empowerment and job embeddedness among nurses under the umbrella of the new Universal Health Insurance in Egypt.

Methods

A descriptive cross-sectional design was employed from August 2025 to November 2025. A total of 213 nurses working at Aswan Specialized Hospital, affiliated with the UHI system in Upper Egypt, were recruited. Data were collected using a demographic questionnaire, and psychological empowerment was measured using the Psychological Empowerment Scale, and job embeddedness was assessed using the Global Job Embeddedness Scale. Descriptive statistics, Pearson correlation analysis, and hierarchical linear regression were used for data analysis.

Results

Among 213 nurses, the mean score of psychological empowerment was reported at high overall levels of 5.75 (SD = 0.77), particularly in the dimensions of competence and meaning, alongside moderately high levels of job embeddedness, with a mean score of 4.76 (SD = 0.89). Psychological empowerment showed a significant positive correlation with job embeddedness (r = 0.512, p < 0.001). In hierarchical regression analysis adjusting for demographic and professional covariates, psychological empowerment emerged as a strong and independent predictor of job embeddedness (β = 0.55, 95% CI: 0.398–0.701, p < 0.001), explaining an additional 16.5% of the variance after controlling for covariates (ΔR2 = 0.165). In adjusted analyses, the covariates were not independently associated with job embeddedness.

Conclusion

Psychological empowerment was positively associated with nurses' job embeddedness in the UHI implementation setting. Higher levels of empowerment-related factors were positively associated with greater embeddedness, suggesting their relevance to workforce engagement and retention during health system reform.

Clinical Relevance

Our study findings highlight that psychological empowerment plays a crucial role in fostering nurses' job embeddedness, which is critical for sustaining workforce retention and stability. Enhancing supportive leadership and nurses' involvement in decision-making is critical, especially during the early phase implementation of the universal health insurance system reform.

Electrical Stimulation of the Nucleus Accumbens for Severe, Refractory Self-Injurious Behaviour in Children (EASE-SIB): protocol for a randomised double-blinded crossover trial

Por: Mithani · K. · Sauter · S. · Hagopian · L. P. · Breitbart · S. · Sriharan · S. · Kisteroff · F. · Huynh · M. · Malik · S. · Thorpe · K. E. · Huber · J. · Gorodetsky · C. · Ibrahim · G. M.
Introduction

Self-injurious behaviour (SIB) consists of persistent, repetitive movements that can result in serious injury without suicidal intent. These behaviours are prevalent among children with neurodevelopmental disorders, including profound autism. Although many individuals benefit from currently available therapies, some exhibit treatment-refractory SIB that necessitates ongoing use of personal protective equipment and restraint, presumably due to stronger neurobiological drivers. We recently completed a phase I, open-label clinical trial demonstrating the safety, feasibility and preliminary efficacy of bilateral deep brain stimulation targeting the nucleus accumbens (NAc-DBS) in children with profound autism and severe, refractory SIB. The objective of the proposed study is to characterise the effectiveness of NAc-DBS in treating severe, refractory SIB in this unique and vulnerable population.

Methods and analysis

A single-centre, randomised double-blinded, crossover trial is proposed. Informed by the results of our pilot study, 25 subjects with autism spectrum disorder and severe, refractory SIB will undergo bilateral NAc-DBS. Following a 4-week recovery period, participants will be randomised to either group A (stimulation ON then OFF) or group B (stimulation OFF then ON). Each block will last 12 weeks, separated by a 2-week washout period. Following completion of the second block, all participants will enter a 6-month open-label phase with stimulation ON. The primary outcome is the difference in the Repetitive Behaviour Scale–Revised total score, between DBS-ON and DBS-OFF conditions. Secondary outcomes include measures of quality of life, caregiver burden, daily logs of SIB events and direct observation of SIB under structured analogues.

Ethics and dissemination

The proposed trial has been approved by the institutional Research Ethics Board (1000081171). Trial results will be disseminated through peer-reviewed publications and conference presentations.

Trial registration number

NCT06529380

Digital Bridges and Emotional Anchors: Nurses Facilitating Social Connectivity in Virtual Age‐Friendly Communities

ABSTRACT

Aim

To explore how nurses facilitate social connectivity among older adults in virtual age-friendly communities in Al-Ahsa, Saudi Arabia by serving as digital bridges and emotional anchors. Additionally, the study aimed to identify the factors that influence the effectiveness of nurse-led interventions in enhancing digital literacy and reducing social isolation among older adults.

Design

A qualitative study.

Method

Semi-structured interviews were conducted between June and August 2024 with 12 older adult participants and 10 nurse participants involved in virtual age-friendly community initiatives in Al-Ahsa, Saudi Arabia. Data were analysed using a hybrid approach that combined deductive thematic analysis—guided by socio-technical systems theory and relationship-centred care principles—with inductive analysis to identify key themes related to digital facilitation, emotional support and social connectivity.

Results

The findings indicate that nurse-led digital and emotional support significantly enhances older adults' digital literacy and emotional well-being, leading to improved social connectivity. Nurses acting as digital bridges provided essential technical guidance, while their role as emotional anchors offered empathetic support that mitigated feelings of isolation. However, challenges such as technical issues, resource limitations and cultural factors moderated the overall effectiveness of these interventions.

Conclusion

The study underscores the pivotal role of nurse-led interventions in promoting social connectivity among older adults in virtual settings. Future research should explore strategies to overcome technical and resource-related barriers and further integrate culturally sensitive approaches to optimise the benefits of digital health initiatives.

Implication for the Profession and/or Patient Care

Healthcare practitioners and policymakers should prioritise the implementation of nurse-led digital and emotional support programmes to reduce social isolation and improve the overall quality of life for older adults. Culturally tailored interventions are essential to address the unique needs of ageing populations in the digital era.

Reporting Method

The study was reported in accordance with the Consolidated Criteria for Reporting Qualitative Research (COREQ).

Patient or Public Contribution

The insights obtained from older adult participants and nurses provided critical understanding of the dynamics and impact of digital and emotional support within virtual age-friendly communities.

Relationship Between Health Literacy, Health Protective Behaviour, Quality of Life and Social Health in Older Adults Living in the Community

ABSTRACT

Aims

The aim of this study was to examine the relationship between health literacy, health protective behaviour, quality of life and social health in older adults living in the community.

Design

A cross-sectional study.

Method

This observational study was carried out by interviewing 600 older adult people living in a province in the South Marmara region of Turkey using a multi-stage cluster sampling method. The dependent variable of the study was social health perception, and multivariate linear regression analysis was used in the analyses. Reporting of the study followed the STROBE checklist.

Results

The social health of the participants is at a medium level. As a result of linear regression analysis, the social health score was found in those who have high school education or higher, those who live with their children and those who live alone to be significantly lower in those with poor general health perception. As age increases, health literacy increases, and quality of life decreases, the social health score decreases (p < 0.05).

Conclusion

In this context, steps should be taken to increase social harmony and social support for the older adult; the perceived environment should be improved, and environments that facilitate the lives of the older adult should be created.

Relevance to Clinical Practice

Determining the level of social health of the older adult and identifying the related factors is important in terms of improving the quality of services to be provided for the older adult. In this context, it is important for health professionals to take initiatives to improve the lifestyle, health literacy and quality of life of the older adult.

Perspectives on multimorbidity care provision among public hospital-based healthcare workers in Blantyre and Chiradzulu, Malawi: A qualitative study

by Gift Treighcy Banda-Mtaula, Ibrahim Simiyu, Sangwani Nkhana Salimu, Stephen A. Spencer, Nateiya M. Yongolo, Marlen Chawani, Hendry Sawe, Jamie Rylance, Ben Morton, Adamson S. Muula, Eve Worall, Felix Limbani, Miriam Taegtmeyer, Rhona Mijumbi, on behalf of the Multilink consortium

Multimorbidity, the presence of multiple chronic health conditions, is a leading cause of death globally. In Malawi, chronic noncommunicable and communicable diseases such as HIV frequently co-exist, putting pressure on an under-resourced system. However, the health system is primarily structured around disease-specific [vertical] programs, which hinders person-centred care approaches to multimorbidity. Our study focuses on multimorbidity care and explores the perceptions of healthcare workers on the patient pathways and service organisation throughout the patient’s interaction with the health facilities. This cross-sectional qualitative study took an interpretivist approach. We conducted 13 days of clinical observations at Queen Elizabeth Central Hospital and Chiradzulu District Hospital. We also conducted 13 days of clinical observations and semi-structured in-depth interviews with different cadres of purposively sampled healthcare workers (n = 22) at Queen Elizabeth Central Hospital and Chiradzulu District Hospital. Through thematic analysis, we identified an understanding of the organisation of care and healthcare workers’ perspectives on the delivery of services. Findings showed both hospitals provided services for inpatients and outpatients with multimorbidity, including screening, management, prevention of secondary conditions and rehabilitation. Patient diagnosis and management for multimorbidity were often delayed due to frequent stockouts of medication and consumables necessary for diagnostic testing for NCDs at the hospital level. Some healthcare workers were not equipped with the knowledge, skills, or guidelines to manage multimorbidity. As HIV care is currently better resourced than other chronic conditions, healthcare facilities may strengthen the supply chain, healthcare workers’ training sessions and monitoring and evaluation tools to ensure NCDs are well managed, learning from HIV programmes.

Postgraduate students’ perceptions of artificial intelligence integration in research: A cross-sectional study

by Ibrahim Naif Alenezi, Fathia Ahmed Mersal, Amal Ahmed Elbilgahy

Background

Generative artificial intelligence (AI) tools such as ChatGPT are increasingly used in academic research, yet evidence on postgraduate students’ perceptions remains limited in non-Western and health-professional contexts. Understanding how students perceive AI’s benefits, risks, and ethical implications is essential for informing institutional research policies.

Methods

This cross-sectional case study surveyed 267 master’s students enrolled in nursing and health profession programs at Northern Border University in Arar, Saudi Arabia. Data were collected between October 1 and November 15, 2025, using a validated 54-item questionnaire that assessed perceived benefits, perceived risks, privacy concerns, mistrust in AI, performance anxiety, social bias, regulatory matters, liability issues, and intention to adopt AI tools. Multiple linear regression with heteroscedasticity-robust (HC3) standard errors was used to identify predictors of AI adoption intention.

Results

Most participants (85.0%) reported prior use of AI tools, predominantly ChatGPT. Perceived benefits were the strongest predictor of intention to adopt AI for research purposes (β = 0.588, p 2 = 0.560).

Conclusions

Among nursing and health profession master’s students at a regional Saudi university, findings indicate pragmatic optimism toward AI integration in academic research, driven primarily by perceived benefits alongside heightened ethical and privacy awareness. Privacy concerns appear to reflect critical literacy rather than barriers to adoption.

Validation of the Workplace COVID-19 Knowledge and Stigma Scale (WoCKSS) in Malaysia: a cross-sectional study using item response theory and factor analyses

Por: Baharuddin · I. H. · Naing · N. N. · Patterson · M. · Yasin · S. M. · Ibrahim · K. · Ismail · N.
Objective

To validate the Workplace COVID-19 Knowledge and Stigma Scale (WoCKSS) using item response theory (IRT), exploratory factor analysis (EFA) and confirmatory factor analysis (CFA).

Design

Cross-sectional psychometric validation.

Setting

Manufacturing companies registered in Malaysia.

Participants

A total of 137 factory workers participated in the exploratory phase and 300 in the confirmatory phase. Inclusion criteria were Malaysian nationality and ability to read Malay.

Methods

The knowledge domain was examined using the two-parameter logistic IRT model in two stages: an exploratory IRT analysis in phase 1 to screen items and a confirmatory IRT analysis in phase 2 to evaluate the final item set. The stigma domain was analysed using EFA followed by CFA. Reliability was assessed using Cronbach’s alpha and McDonald’s omega (). The development process and content and face validity results were previously published.

Results

14 knowledge items were retained after exploratory IRT and formed the final knowledge scale evaluated in confirmatory IRT. For these final items, discrimination parameters ranged from 0.77 to 3.17 and difficulty values from –4.47 to 0.23, with unidimensionality supported (p=0.644). EFA supported a three-factor stigma structure (stereotype, fear, prejudice), and CFA confirmed excellent model fit (2=8.91, df=11, p=0.630; root mean square error of approximation=0.00; Comparative Fit Index=1.00; Tucker-Lewis Index=1.00; standardised root mean square residual=0.021). Composite reliability by McDonald’s omega ranged from 0.691 to 0.893.

Conclusion

WoCKSS is a reliable and valid instrument for assessing workplace COVID-19 knowledge and stigma in industrial sectors in Malaysia.

Exploring society‐to‐cells resilience among community members: A multidisciplinary study in outpatient clinics with diverse participant demographics

Abstract

Aim

This study explores and understands community members' resilience in outpatient clinics, considering various dimensions and types of resilience.

Design

A cross-sectional study.

Methods

This study was conducted in Saudi outpatient clinics from September to December 2023 and included 384 individuals chosen through systematic random sampling. Various tools were used, such as Social Cohesion and Trust Scale, Community Resilience Assessment Tool, Community Assessment of Psychic Experiences, Environmental Resilience Assessment, Economic Resilience Index, Connor-Davidson Resilience Scale, Brief Resilience Scale, Resilience Scale for Adults and Healthcare Resilience Index.

Results

Participants displayed a robust overall resilience level, as indicated by Total Connor–Davidson Resilience Scale score of 63.0 ± 9.0. Additionally, they demonstrated commendable levels of resilience in Total Brief Resilience Scale (56.04 ± 8.6), Resilience Scale for Adults (82.5 ± 7.2) and Healthcare Resilience Index (45.8 ± 5.5). These findings offer significant insights into psychological and emotional well-being of the study population, highlighting their adaptive capacities and coping mechanisms across various life domains.

Conclusion

This study provides valuable insights into the multidimensional nature of resilience in outpatient settings. The cross-sectional design sets the groundwork for future longitudinal investigations, highlighting the need for a holistic approach to understanding and promoting resilience.

Impact

This study holds immediate implications for participants and their communities. It underscores the adaptive capacities and coping mechanisms prevalent in the outpatient population by revealing commendable resilience levels. This insight enhances individuals' psychological and emotional well-being, contributing positively to the overall resilience and communal strength. Additionally, this study sheds light on how resilience among community members in Saudi Arabia relates to international advanced nursing communities, providing insight into their work.

Patient or Public Contribution

Patients who have received outpatient services in the past 6 months were purposively chosen to ensure a diverse representation across age, gender and socio-economic backgrounds in this study.

Contraceptive use intention among women in Pakistan: Application of theory of planned behavior

by Zoya Waqas, Aisha Irum, Muhammad Ibrahim, Maheen Sughra, Sanaa Khan, Ayesha Khan, Adnan Ahmad Khan

Introduction

Unintended pregnancies remain a major public health concern globally and in Pakistan, where family planning (FP) uptake continues to be hindered by entrenched social and behavioral barriers. This study applies the Theory of Planned Behavior (TPB) to examine how attitudes, subjective norms, and perceived behavioral control (PBC) shape women’s contraceptive intentions in Pakistan.

Methods

We analyzed data from 13,335 non-pregnant women aged 15–49 using the Pakistan Demographic and Health Survey (PDHS) 2017–18. Partial Least Squares Structural Equation Modeling (PLS-SEM) was used to test TPB pathways. Model reliability, validity, and fit were assessed using Composite Reliability, Average Variance Extracted, discriminant validity indices, and bootstrapped estimates to ensure analytical rigor.

Results

Over half of respondents lacked formal education, and most (84%) were unemployed. Only 23% had FP knowledge, and 96% were unaware of contraceptive side effects. Subjective norms negatively influenced contraceptive intentions (β = −0.056, p  Conclusion

The study demonstrates the applicability of TPB for understanding contraceptive intentions in a collectivist, patriarchal context. Subjective norms and PBC emerge as critical determinants, underscoring the need for interventions that engage families, strengthen women’s autonomy, and improve access to FP services. The findings offer a theoretically grounded and policy-relevant framework for designing behaviorally informed family planning programs in Pakistan.

Bridging the stroke care gap: development and validation of CaknaStrok Education Package (CEP) for caregivers of stroke survivors in Malaysia

Por: Sidek · N. N. · Kamalakannan · S. · Musa · K. I. · Long Tuan Kechik · T. S. M. · Hamzah · N. · Abd Hamid · R. I. · Darus · D. · Ibrahim · K. A. · Seman · N. · Ahamad Fouzi · L. · Abdul Hamid · F. · Ismail · T. A. T.
Background

Stroke is one of the top causes of disability in Malaysia, yet caregivers have limited access to structured, culturally tailored education to support poststroke care.

Objectives

To develop and validate the CaknaStrok Education Package (CEP), a blended learning intervention comprising a printed guidebook and a trilingual mobile health application for informal stroke caregivers in Malaysia.

Design

Methodological study involving the development and validation of a caregiver education programme guided by the Analyse, Design, Develop, Implement, Evaluate (ADDIE) instructional design framework.

Setting

Development and validation were conducted in Malaysia between January 2022 and December 2023. Both experts and caregivers were recruited from two tertiary hospitals on the East Coast of Malaysia, with caregivers identified from inpatient wards and outpatient clinics at these hospitals.

Participants

Content validation involved 10 multidisciplinary experts. Face validation involved 14 informal stroke caregivers who met eligibility criteria, and all completed the study.

Methods

CEP was developed based on prior needs assessment and expert input. Content validation was undertaken using the Content Validity Index (CVI) and face validation using the Face Validity Index (FVI), both assessed on a four-point Likert scale. Qualitative feedback was also obtained from the participants.

Results

CEP consists of six modules delivered via a printed guidebook and a trilingual app with videos, assessment tools and local resources. Experts rated the content highly valid (Scale-level (S)-CVI/the average method (Ave): 0.97–0.99 across domains). Caregivers reported strong acceptability (S-FVI/Ave: 0.95–0.99). Qualitative feedback from experts and caregivers informed refinements to content clarity, usability and presentation, including improved navigation, consistent language use and enhanced visual design. Suggestions requiring substantial structural changes were documented for future iterations.

Conclusions

The CEP shows strong content and face validity as a blended caregiver education tool. By combining printed and digital formats, CEP addresses cultural and access challenges and provides a scalable model for stroke caregiver education in Malaysia. Further pilot or feasibility studies are warranted to evaluate usability, engagement and implementation in real-world settings prior to effectiveness evaluation.

Efficacy of Digital Mental Health Interventions for Depression and Anxiety in Older Adults: A Systematic Review and Meta‐Analysis

ABSTRACT

Background

Older adults face growing risks of depression and anxiety, yet stigma, comorbidities, cost, and limited access impede receipt of conventional care. Digital mental health interventions (DMHIs), including immersive virtual reality (VR), exergaming, and mobile apps, may reduce these barriers.

Aim

To evaluate the efficacy of DMHIs in reducing depressive and anxiety symptoms among adults aged ≥ 50 years.

Methods

We conducted a PRISMA adherent systematic review and meta-analysis of randomized controlled trials. Interventions included immersive VR, exergaming/physical digital platforms, mobile applications, and digital cognitive training. Standardized mean differences (SMDs) were pooled with random effects models; heterogeneity was assessed with I 2.

Results

Nineteen RCTs (n = 718; mean ages 50.9–84.7 years) met inclusion criteria. Across studies, DMHIs significantly reduced depressive symptoms (SMD = −0.656, 95% CI = −0.932 to −0.380; p < 0.001) and anxiety symptoms (SMD = −0.559, 95% CI = −0.740 to −0.380; p < 0.0001). Immersive and physically engaging modalities (e.g., VR, exergaming) outperformed app-based approaches. Heterogeneity ranged from moderate to high (I 2 ≈ 69.6%–97%).

Linking Evidence to Action

Offer DMHIs: especially VR or exergaming when access to in-person therapy is limited or as an adjunct to usual care. Provide brief onboarding and, when feasible, caregiver support to boost adherence and confidence with technology. Select or configure age-friendly interfaces (e.g., large fonts, simple navigation) to address common usability barriers. Integrate DMHIs into stepped-care or rehabilitation pathways and monitor outcomes with validated tools (e.g., GDS, STAI). Address equity by supplying devices/connectivity solutions and consider cost-effectiveness and long-term engagement in implementation plans.

Trial Registration: PROSPERO ID: CRD420250655153

Family functionality and its association with non-communicable diseases among urban adults in Selangor, Malaysia: a cross-sectional study

Por: Abdullah · N. N. · Mohammed Ali Azzani · M. · Mohamad · M. · Ismail · Z. · Jamil · A. T. · Isa · M. R. · Yasin · S. M. · Suddin · L. S. · Ibrahim · K. · Selamat · M. I. · Azhar · Z. I. · Ismail · N. · Ahmad Saman · M. S. · Xin Wee · C. · Samsudin · E. Z. · Muzaini · K. · Yaacob · S. S.
Objectives

Non-communicable diseases (NCDs) are rapidly escalating in developing countries and social factors such as the dynamics of the family play an important part in the lifestyle choices that lead to the onset and maintenance of chronic illness. There remains a gap in Malaysia as the majority of the studies were focused on the normal population rather than directly towards persons having NCDs. This study aimed to examine emerging risk factors such as family functionality and its association with NCD.

Design

A cross-sectional survey was conducted using a multistage random sampling method.

Setting

Urban residential areas in Selangor, Malaysia.

Participants

A total of 2542 adults residing in urban areas of Selangor were recruited.

Primary and secondary outcome measures

Family functionality was measured using the APGAR (Adaptation, Participation, Gain or Growth, Affection and Resources) scale and multiple logistic regression was performed to measure the association between emerging risk factors and NCD.

Results

The prevalence of diabetes mellitus and hypertension was 10.8% and 6.1%, respectively. Widowed/separated status (adjusted OR (AOR) 41.53, 95% CI 19.06 to 90.48, p value=0.001) was reported to be a predictor of diabetes. As for hypertension, familial functionality (AOR 4.2, 95% CI 1.11 to 14.50, p value

Conclusions

There is a growing concern that family functionality is an emerging risk factor for NCDs. Future family-centred health promotion programmes should be incorporated to improve self-management behaviours and health outcomes.

Assessing stress restorative potential of plant species richness and plant landscape types of pocket parks: The mediating role of aesthetic quality

by Yu Wang, Filzani Illia Ibrahim, Junlin Chang, Siti Norzaini Zainal Abidin

The issue of stress among urban residents is becoming increasingly serious, affecting both physical and mental health in cities in China. Pocket parks serve as essential green spaces for people’s well-being in high-density urban environments. However, limited empirical research has examined how plant landscape components support stress recovery among urban residents, particularly in the context of pocket parks in China. This study investigated the effects of plant species richness and plant landscape types on stress recovery, with a focus on the mediating role of perceived aesthetic quality. A pre-test and post-test design were conducted in six selected pocket parks with different landscape characteristics using questionnaire surveys. A total of 605 urban residents were recruited using a random sampling method at different sites. The results indicated that medium plant species richness (t = −10.502, p p 

Beyond the counter: Pharmacists’ preparedness and response strategies in terrorism-related emergencies in Quetta, Pakistan

by Fahad Saleem, Fazal ur Rehman Khilji, Sajjad Haider, Qaiser Iqbal, Baharudin Ibrahim, Fatiha Hana Shabaruddin, Mohammad Bashaar

Terrorism-related disasters (TRDs) continue to exert profound and recurring pressures on healthcare systems, particularly in vulnerable regions like Pakistan. Although pharmacists are increasingly recognized as an essential component of disaster management, there is a clear gap in the literature regarding their preparedness, experience, and specific roles in responding to TRDs particularly in low and middle-income countries. This study aimed to explore the preparedness, experiences, and response strategies of pharmacists managing TRDs at the Trauma Centre of Sandeman Provincial Hospital, Quetta, Pakistan. A qualitative design was adopted, guided by the Consolidated Criteria for Reporting Qualitative Research. Semi-structured, face-to-face interviews were conducted with pharmacists (n = 10) providing services at the Trauma Centre. Data were audio-recorded, transcribed verbatim, validated by participants, and analyzed using thematic content analysis. Analysis revealed five overarching themes: (1) pharmacists’ experiences with terrorism-related incidents and existing response mechanisms; (2) professional and personal responses to emergencies, reflecting both commitment and psychological burden; (3) preparedness challenges, including lack of disaster management training, limited awareness of policies and protocols, and inadequate understanding of triage and coordination; (4) barriers such as security risks, pharmacy curriculum deficiencies, insufficient experiential learning, and minimal involvement in planning and management activities; and (5) recommendations for strengthening capacity, including revising curricula, implementing structured training programs, conducting regular disaster drills, and expanding pharmacists’ roles in preparedness and response. Findings revealed a pronounced lack of formal training in disaster management, limited awareness of protocols and triage systems, and minimal involvement of pharmacists in planning and coordination activities. Despite strong professional commitment and frontline engagement, pharmacists’ contribution remain constrained by educational, structural, and policy-level shortcomings. The study highlights the urgent need for integrating disaster management into pharmacy curricula, implementing structured training programs and regular disaster drills, and expanding pharmacists’ roles within institutional and national disaster preparedness frameworks.

Does road safety cointegrate with socio-economic conditions in rich developing countries?

by Ibrahim Abdalla Alfaki, Michal Grivna, Mohamed El Sadig

Despite significant progress in road safety in developed countries, it remains a persistent and critical challenge in the developing world. This study investigates the long- and short-term relationships between socio-economic conditions and road safety performance in affluent developing countries, using the United Arab Emirates (UAE) as a case study. Employing an autoregressive distributed lag (ARDL) cointegration error-corrected model with data from 1980 to 2024 (sourced from the UAE Federal Government, the World Bank, and UN World Population Prospects), the analysis examines the link between the road crash severity index (fatalities to total injuries) and core socio-economic variables—GDP per capita, unemployment rate, and population density—while controlling for traffic law enforcement via fines. The findings confirm a long-term equilibrium, with an error correction term indicating road safety adjusts to socio-economic shocks at a rapid annual rate of 60%. Granger-causality tests further establish that these socio-economic factors significantly influence road safety outcomes, a concern underscored by an identified upward trend in crash severity. We conclude that socio-economic conditions are a fundamental determinant of road safety, highlighting the necessity for policy interventions that move beyond traditional engineering solutions. Consequently, road safety must be reframed not solely as a transportation concern but as an integral objective of public health and socioeconomic policy, which requires a collaborative, multi-sectoral approach to forge a resilient, safe system.

Association between depression, anxiety and loneliness with internet addictions among public university students in Malaysia

Por: Muhamad · N. A. · Maamor · N. H. · Tengku Baharudin Shah · T. P. N. · Jamalluddin · N. H. · Leman · F. N. · Rosli · I. A. · Nik Azhan · N. A. F. · Chemi · N. · Muhamad Rasat · M. A. · Abdullah · N. · Abdul Jabbar · N. · Ibrahim · N. · Mohamad Isa · M. F.
Objective

To determine the prevalence of internet addiction and examine its association with psychological factors specifically depression, anxiety and loneliness among Malaysian public university students.

Design

Cross-sectional study.

Setting

All public universities in Malaysia, 20 universities.

Participants

The study included 7278 students from 20 public universities in Malaysia.

Main outcome measures

Statistical analyses were performed usingSTATA V.17 software. Descriptive statistics summarised participants’ demographic characteristics, prevalence of internet addiction and psychological distress (depression, anxiety and loneliness). Pearson’s correlation was used to assess bivariate relationships between internet addiction and psychological variables, while multiple logistic regression identified independent factors associated with internet addiction after adjusting for significant confounders.

Results

The study found that 38.6% of the students showed signs of internet addiction, along with a high level of psychological distress; 24.8% had depressive symptoms, 32.4% experienced anxiety and 35.5% reported loneliness. Moderate positive correlations were observed between internet addiction and depression, anxiety and loneliness (p

Conclusion

The findings indicate significant associations between internet addiction and psychological factors such as depression, anxiety and loneliness. A comprehensive, multifaceted approach is essential to address psychological distress among university students and reduce the risk of internet addiction.

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